New KHV detection technique developed


Editor's Picks
Do I need an aquarium filter
Features Post
Do I need a filter for an aquarium?
07 February 2024
Features Post
How to set up an African biotope aquarium
01 February 2024
Fishkeeping News Post
AQUAH: A new UK aquatic and reptile show for 2024
17 January 2024
Practical Fishkeeping Readers' Poll 2023
Fishkeeping News Post
Readers' Poll 2023
07 August 2023

Japanese scientists have developed a new technique for detecting Koi Herpes Virus which is claimed to be as effective as PCR-based tests.

Gunimaladevi and co-workers have developed an experimental procedure called loop-mediated isothermal amplification (LAMP) which they describe in the latest issue of the Journal of Fish Diseases.

LAMP amplifies the tiny quantities of viral DNA with high specificity and works very quickly under isothermal (constant temperature) conditions.

The workers claim that their protocol for detecting KHV with LAMP, which uses markers from the thymidine kinase (tk) gene of KHV, has similar limits to detection protocols utilising the polymerase chain reaction (PCR).

For more details see the paper: Gunimaladevi I, Kono T, Venugopal MN, Sakai M. (2004) - Detection of koi herpesvirus in common carp, Cyprinus carpio L., by loop-mediated isothermal amplification. J Fish Dis. 2004 Oct;27(10):583-9.